Publications

2024

Meena, J.K.; Wang, J.H.; Neill, N.J.; Keough, D.; Putluri, N.; Katsonis, P.; Koire, A.M.; Lee, H.; Bowling, E.A.; Tyagi, S.; Orellana, M.; Dominguez-Vidana, R.; Li, H.; Eagle, K.; Danan, C.; Chung, H.C.: Yang, A.D.; Wu W.; Kurley, S.J.; Ho, B.M.; Zoeller, J.R.; Olson, C.M.; Meerbrey, K.L.; Lichtarge, O.; Sreekumar, A.; Dacso, C.C.; Guddat, L.W.; Rejman, D.; Hockova, D.; Janeba, Z.; Simon, L.M.; Lin, C.Y.; Pillon, M.C.; Westbrook, T.F. MYC Induces Oncogenic Stress Through RNA Decay and Ribonucleotide Catabolism in Breast Cancer. Cancer Discovery. (2024) 14: 1699-1716.

2022

Schellenberg, M.J.*; Maheshwari, S.; Pillon, M.C.* (2022) Recombinant Protein Production Using Mammalian Expression Systems. In R. Bradshaw, P. Stahl, G. Hart (Ed). Encyclopedia of Cell Biology. Second Edition. (Invited book chapter) *Co-corresponding authors

2021

Frazier, M.N.; Dillard, L.B.; Krahn, J.M.; Perera, L.; Williams, J.G.; Wilson, I. M.; Stewart, Z.D.; Pillon, M.C.; Deterding, L.J.; Borgnia, M.J.; Stanley, R.E. Characterization of SARS2 Nsp15 Nuclease Activity Reveals it’s Mad About U. Nucleic Acids Research. (2021) 49: 10136-10149.

Pillon, M.C.*,§; Frazier, M.N.§; Dillard, L.B.§; Williams, J.G.; Kocaman, S.; Krahn, J.M.; Perera, L.; Hayne, C.K.; Gordon, J.; Stewart, Z.D.; Sobhany, M.; Deterding, L.J.; Hsu, A.L.; Dandey, V.P.; Borgnia, M.J.; Stanley, R.E.* Cryo-EM Structures of the SARS-CoV-2 Endoribonuclease Nsp15. Nature Communications. (2021) 12: 636. §Authors contributed equally. * Co-corresponding authors.

2020

Pillon, M.C.*; Gordon, J.; Frazier, M.N.; Stanley, R.E*. HEPN RNases – An Emerging Class of Functionally Distinct RNA Processing Enzymes. Critical Reviews in Biochemistry and Molecular Biology. (2020) 1-30. DOI: 10.1080/10409238.2020.1856769 *Co-corresponding authors.

Frazier, M.N; Pillon, M.C.; Kocaman, S.; Gordon, J.; Stanley, R.E. Structural Overview of Macromolecular Machines Involved in Ribosome Biogenesis. Current Opinion in Structural Biology. (2020) 67: 51-60.

Pillon, M.C.§; Goslen, K.H.§; Gordon, J.; Wells, M.L.; Williams, J.G.; Stanley, R.E. It takes two Las1 HEPN endoribonuclease domains to cut the RNA correctly. The Journal of Biological Chemistry. (2020) 295: 5857-5870. §Authors contributed equally.

Pillon, M.C.; Stanley, R.E. Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates. J. Vis. Exp. (2020) 159: 10.3791/61258.

2019

Pillon, M.C.; Hsu, A.L.; Krahn, J.M.; Williams, J.G.; Goslen, K.H.; Sobhany, M.; Borgnia, M.J.; Stanley, R.E. Cryo-EM Reveals Active Site Coordination Within a Multienzyme pre-rRNA Processing Complex. Nat. Struct. Mol. Biol. (2019) 26: 830-839.

Pillon, M.C.; Lo, Y.H.; Stanley, R.E. IT’S 2 for the Price of 1: Multifaceted ITS2 Processing Machines in RNA and DNA Maintenance. DNA Repair. (2019) 81: 102653.

Gordon, J.; Pillon, M.C.*; Stanley, R.E.* Nol9 is a Spatial Regulator for the Human ITS2 pre-rRNA Processing Complex. J. Mol. Biol. (2019) 431: 3771-3786.* Co-corresponding authors.

Almawi, A.W.; Scotland, M.K.; Randall, J.R.; Liu, L.; Martin, H.K.; Sacre, L.; Shen, Y.; Pillon, M.C.; Simmons, L.A.; Sutton, M.D.; Guarné, A. Binding of the regulatory domain of MutL to the sliding b-clamp is species specific. Nucleic Acids Res. (2019) 47: 4831-4842.

Mok, M.C.Y.; Campalans, A.; Pillon, M.C.; Guarné, A.; Radicella, J.P.; Junop, M. Identification of an XRCC1 DNA binding activity essential for retention at sites of DNA damage. Scientific Reports. (2019) 9: 3095.

Liu, L.; Ortiz Castro, M.C.; Rodríguez González, J.; Pillon, M.C.; Guarné, A. The Endonuclease Domain of Bacillus subtilis MutL is Functionally Asymmetric. DNA Repair. (2019) 73: 1-6.

2018

Pillon, M.C.; Sobhany, M. and Stanley, R.E. Characterization of the Molecular Crosstalk within the Essential Grc3/Las1 pre-rRNA Processing Complex. RNA. (2018) 24: 721-738.

Lo, Y.H.; Pillon, M.C.; Stanley, R.E. Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. cerevisiae. J. Vis. Exp. (2018) 131: e56953.

Pillon, M.C. and Stanley, R.E. Nuclease integrated Kinase Super Assemblies (NiKs) and Their Role in RNA Processing. Curr. Genetics. (2018) 64: 183-190.

2017

Pillon, M.C. and Guarné, A. Complementary uses of small angle X-ray scattering and X-ray crystallography. Biochim Biophys Acta Proteins Proteom. (2017) 1865: 1623-1630.

Pillon, M.C.; Sobhany, M.; Borgnia, M.J.; Williams, J.G.; Stanley, R.E. Grc3 programs the essential endoribonuclease Las1 for specific RNA cleavage. Proc. Natl. Acad. Sci. USA. (2017) 114: E5530-E5538.

Lo, Y.H; Romes, E.; Pillon, M.C.; Sobhany, M; Stanley, R.E. Structural analysis reveals features of ribosome assembly factor Nsa1/WDR74 important for localization and interaction with Rix7/NVL2. Structure. (2017) 25: 762-772.

2016

Wan, L.C.K.; Pillon, M.C.; Thevakumaran, N.; Sun, Y.; Chakrabartty, A.; Guarné, A.; Kurinov, I.; Durocher, D.; Sicheri, F.  Structural and functional characterization of KEOPS dimerization by Pcc1 and its role in t6A biosynthesis. Nucleic Acids Res. (2016) 19: 6971-6980.

Stogios, P.J.; Cox, G.; Spanogiannopoulos, P.; Pillon, M.C.; Waglechner, N.; Skarina, T.; Koteva, K.; Guarné, A.; Savchenko, A. and Wright, G.D. Rifampin phosphotransferase is an unusual antibiotic resistance kinase. Nature Commun. (2016) 22: 11343-11354.

Lenhart, J.S.; Pillon, M.C.; Guarné, A.; Biteen, J.S. and Simmons, L.A. Mismatch repair in Gram-positive bacteria. Res. Microbiol. (2016) 167: 4-12.

2015

Pillon, M.C.; Babu, V.M.P., Randall, J.R., Cai, J., Simmons, L.A., Sutton, M.D. and Guarné, A. The sliding clamp tethers the MutL endonuclease domain to DNA. Nucleic Acid Res. (2015) 43: 10746-10759.

Zeqiraj, E.; Tian, L.; Piggott, C.A.; Pillon, M.C.; Duffy, N.M.; Ceccarelli, D.F.; Keszei, A.F.; Lorenzen, K.; Heck, A.J.R.; Kurinov, I.; Orlicky, S.; Guarné, A.; Greenberg, R.A. and Sicheri, F. Higher order assembly of BRCC36-KIAA0157 is required for DUB activity and biological function. Mol Cell. (2015) 59: 970-983.

2014

Huang, H.; Zeqiraj, E.; Dong, B.; Kant Jha, B.; Duffy, N.; Orlicky, S.; Thevakumaran, N.; Talukdar, M.; Pillon, M.C.; Ceccarelli, D.F.; Wan, L.; Juang, Y.; Mao, D.Y.L.; Gaughan, C.; Brinton, M.A.; Perelygin, A.A.; Kourinov, I.; Guarné, A.; Silverman, R.H.; Sicheri, F. Dimeric structure of pseudokinase RNase L bound to 2-5A reveals a basis for interferon induced antiviral activity. Mol Cell. (2014) 53: 221-234.

2013

Lenhart, J.S.§; Pillon, M.C.§; Guarné, A.; and Simmons, L.A. Trapping and visualizing intermediate steps in the mismatch repair pathway in vivo. Mol. Microbiol. (2013) 90: 680-698. §Authors contributed equally.

Pillon, M.C.; Dubinsky, M.; Johnston, R.N.; Liu, S.L. and Guarné, A. Characterization of the defects in the ATP lid of E. coli MutL that cause transient hypermutability. DNA Repair. (2013) 12: 864-869.

2011

Pillon, M.C.; Miller, J.H.; and Guarné, A. The endonuclease domain of MutL interacts with the β sliding clamp. DNA Repair. (2011) 10: 87-93.

2010

Pillon, M.C.; Lorenowicz, J.J.; Ukelmann, M.; Klocko, A.D.; Mitchell, R.R.; Chung, Y.S.; Modrich, P.; Walker, G.C.; Simmons, L.A.; Friedhoff, P.; and Guarné, A. Structure of the endonuclease domain of MutL: unlicensed to cut. Mol. Cell. (2010) 39: 145-151.